Lilo ti a pinnu
Apo Idanwo Antibody IgG/IgM (Immunochromatographic Assay) gba ọna goolu colloidal lati ṣe idanimọ ọlọjẹ ti bacillus typhoid (lipopolysaccharide antigen ati antigen amuaradagba awo ode) ninu omi ara / pilasima, eyiti o dara fun ayẹwo akọkọ iranlọwọ ti ikolu typhoid .
Ilana Idanwo
Apo Idanwo Antibody IgG/IgM Typhoid (Imunochromatographic Assay) jẹ ajẹsara chromatography sisan ti ita.Kasẹti idanwo naa ni: 1) paadi conjugate awọ burgundy kan ti o ni atunda S. typhoid H antigen ati O antigen ti a so pọ pẹlu goolu colloid (Typhoid conjugates) ati ehoro IgG ‐gold conjugates, 2) awo awọ nitrocellulose ti o ni awọn ẹgbẹ idanwo meji ninu (IgG) ati awọn ẹgbẹ IgM) ati ẹgbẹ iṣakoso kan (ẹgbẹ C).Ẹgbẹ IgM ti wa ni iṣaju pẹlu monoclonal anti-eda eniyan IgM fun wiwa IgM anti-S.typhi, IgG band ti wa ni kọkọ-ti a bo pẹlu reagents fun awọn erin ti IgG anti-S.typhi , ati awọn C band ti wa ni lai-bo pẹlu ewurẹ egboogi ehoro IgG.
Awọn ohun elo / pese | Opoiye(1 Idanwo/Apo)
| Iwọn (Awọn idanwo 5/Apo)
| Opoiye(Awọn idanwo/Apo 25)
|
Idanwo Apo | 1 idanwo | 5 idanwo | 25 igbeyewo |
Ifipamọ | 1 igo | 5 igo | 25/2 igo |
Sisọ silẹ | 1 nkan | 5 nkan | 25 nkan |
Bag Transport Apeere | 1 nkan | 5pcs | 25 awọn kọnputa |
Isọnu Lancet | 1 nkan | 5pcs | 25 awọn kọnputa |
Awọn ilana Fun Lilo | 1 nkan | 1 nkan | 1 nkan |
Iwe-ẹri Ibamu | 1 nkan | 1 nkan | 1 nkan |
Gba Serum eniyan/Plasma/Eje gbogbo daradara.
1. Yọ tube ayokuro lati inu ohun elo ati apoti idanwo lati inu apo fiimu nipasẹ yiya ogbontarigi.Ṣii kaadi ayẹwo aluminiomu apo bankanje.Yọ kaadi idanwo naa ki o si gbe wọn si ita lori tabili.
2. Lo pipette isọnu, gbigbe 4μL omi ara (tabi pilasima), tabi 4μL gbogbo ẹjẹ sinu ayẹwo daradara lori kasẹti idanwo naa.
3.Open tube saarin nipa lilọ si pa awọn oke.Fi 3 silė (nipa 80 μL) ti diluent assay sinu diluent assay daradara ni apẹrẹ yika.Bẹrẹ kika.
Ka abajade ni iṣẹju 15.Awọn abajade lẹhin iṣẹju 20 ko wulo.
Abajade odi
Nikan laini iṣakoso didara C yoo han ati awọn laini wiwa G ati M ko ṣe afihan, o tumọ si pe ko si ọlọjẹ Typhoid ti a rii ati abajade jẹ odi.
Esi Rere
Ti laini iṣakoso didara mejeeji C ati laini wiwa M yoo han = a rii antibody Typhoid IgM, ati pe abajade jẹ rere fun antibody IgM.
Ti laini iṣakoso didara mejeeji C ati laini wiwa G han = a ti rii atako Typhoid IgG ati abajade jẹ rere fun egboogi IgG.
Ti laini iṣakoso didara C mejeeji ati awọn laini wiwa G ati M han = a rii Typhoid IgG ati IgM aporo, ati abajade jẹ rere fun mejeeji IgG ati IgM aporo.
Abajade ti ko tọ
Ti laini iṣakoso didara C ko ba le ṣe akiyesi, awọn abajade yoo jẹ asan
laibikita boya ila idanwo kan fihan, ati pe idanwo naa yẹ ki o tun ṣe.
Orukọ ọja | Ologbo.Rara | Iwọn | Apeere | Igbesi aye selifu | Trans.& Sto.Iwọn otutu. |
Àjàkálẹ̀ àrùn Ohun elo idanwo Antibody IgG/IgM (Ayẹwo Immunochromatographic) | B023C-01 | 1 igbeyewo / kit | Omi ara/Plasma/Ẹjẹ Gbogbo | 18 osu | 2-30℃ / 36-86℉ |
B023C-05 | 5 igbeyewo / kit | ||||
B023C-25 | 25 igbeyewo / kit |